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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 8    
Website: http://www.ijser.org
scirp IJSER >> Volume 3,Issue 8,August 2012
Characterization and Identification of A ctinomycetes Isolated From Contaminated Soil in Riyadh
Full Text(PDF, )  PP.147-154  
Author(s)
Al-Kadeeb, Siham, A . Al-Rokban ,Ahlam ,H. and Awad, F
KEYWORDS
Aluminum, silver and cobalt resistant actinomycetes, Characterization and Identification of actinomycetes , Riyadh, Saudi Arabia.
ABSTRACT
In this study, twenty soil samples were collected to isolate heavy metals resistant actinomycetes. The collection sites were chosen toward the four geographical directions, east, west, south and north of the gold factory in the second industrial city in Riyadh region at Saudi Arabia. Results has been shown that the best growth medium for actinomycetes isolation was the Agar-Albumin medium. From soil samples collected six isolates were able to grew in media containing 1 mM/I00 ml of either aluminum, silver or cobalt ions .All isolates were found to be belonging to the genus Streptomyces. The isolates were classified on the basis of color into four strains. The first isolate was characterized by white color chain and was identified as S. albus, two isolates has gray color chain and were scored as S. diastaticus and S. atroolivaceous, those with red chain and comprised two isolates ,they were identified as S. violaceus and S. exfoliate and one isolate with yellow color chain identified as S. niveus. The recorded results showed that S. diastaticus and S. albus were the most tolerant actinomycetes to the concentration 1 mM/25 ml medium of aluminum, silver and cobalt.
References
[1] Srivastava, N. K. and Majumder, C. B. Novel Biofiltration Methods for the Treatment of Heavy Metals from Industrial Wastewater J. of Hazardat. M. 151 (1): 1- 8,2008.

[2] Castro-Silva, M. A.; de Souza Lima,A. O.; Gerchenski, A. V.; Jaques,D. B.; Rodrigues, A. l.; de Souza, P.L.; Rörig, L. R Braz. Heavy Metal Resistance of Microorganisms Isolated from Coal Mining Environments of Santa Catarina J. Microbiol. vol.34 suppl.1 :45-47,2003.

[3]Albarracin, V. H.; Amoroso, M. J. and Abate, C. M. Isolation and Characterization of Indigenous Copper- Resistant Actinomycete Strains Chem. der Erde- Geochem. 65 (1): 145-156,2005.

[4] Polti, A.; Amoroso, M. J. and Abate, C. M. Chromium (VI)Resistance and Removal Actinomycete Strains Isolated from Marta Sediments Chem. 67 (4): 660- 667,2007.

[5] Schmidt A, Schmidt A, Haferburg G, Kothe E. Superoxide Dismutases of Heavy Metal Resistant Streptomycetes. J. of Basic Microbiol. 47(1):56-62filter your current search,2007.

[6] Al-Kadeeb, Siham, A . Al-Sugiran ,Nada, I. and Awad, F. Characterization and Identification of Lead, Copper and Mercury Resistance Actinomycetes Isolated from Second Industrial City in Riyadh. Saudi J,of Biol, Sci. 16 (3):41- 61,2009.

[7] Zhao XQ, Li WJ, Jiao WC, Li Y, Yuan WJ, Zhang YQ, Klenk HP, Suh JW, Bai FW. Streptomyces xinghaiensis sp. nov., Isolated from Marine Sediment Int. J ,Syst. Evol. Microbiol. 59(Pt 11):2870-4, 2009.

[8] Pridham, T.G. and Lyons, J. Streptomyces albus (RossiDoria) Waksma,S.A. and Henerici, A.T.: Taxonomic Study of Strains labeled Streptomyces albus. J. Bacteriol. 81: 431-441,1961.

[9] Shirling, E.B. and Gottlieb, D. Methods for Characterization of Streptomycetes Species. Int. J. Systematic Bacteriology . 16 pp. 313,1966.

[10] Pridham, T.G. and Gottlieb, D. The Utilization of Carbon Compounds by Some Actinomycetales as anAid for Species Determination J. Bacteriol. 56: 107-114, 1958.

[11] Pridham, T.G.; Anderson, P.; Foley, G.; Lindenfelser, L.A.; Hesseltine, C.W. and Benedict, R.G.A.A selection of Media for Maintenance and Taxonomic Study of Streptomyces Annual Medical Encyclopedia. Inc. New York ,947-953, 1957.

[12]Waksman, S.A. The Actinomycetes. “A summary of current knowledge”. Ronald Press, New York, 1967.

[13]Waksman, S.A. Strain Specificity and Production of Antibiotic Substances, Characterization and Classification of Species within the Streptomyces griseus Proc. Natl. Acad. Sci. U.S.A. 45, pp. 1043, 1959.

[14] Kuster, E.Outline of Acomparative Study of Criteria Used in the Characterization of Actinomycetes Int. Bull. Bact. Nomen. 2:98-104, 1959.

[15] Shinobu, R. Physiological and Culture Study for Identification of Soil Actinomycetes Species Mem. Osaka, B. Nat. Sci. 7: 1, 1958.

[16] Suter, M., Isolierung und Charakterisierung von MelaninNegativen Mutantenaus Streptomyces glaucescens Ph.D.

Dissertation, Nr. 6276, ETH,Zurich. 1978. [17] Tresner, H.D. and Danga, F., J. Hydrogen Sulphide Production by Streptomyces as Acriterion for Species Differentiation Bact. 75: 239-244,1958.

[18] Harborne, J.B. Phytochemical Methods. ed. Capman and Hall, London,1973.

[19] Becker, B.; Lechevalier, M.P. and Lechevalier, H.A. Chemical Composition of Cell Wall Preparations from Strains of Various form-genera of Aerobic Actinomycetes Appl. Microbiol. 13: 236-243, 1964.

[20] Tresner, H.D.; Davis, M.C. and Backus, E.J. Electron Microscopy of Streptomyces Spore Morphology and its Role in the Species Differentiation J. Bacteriol. 81(1): 70- 80,1961.

[21] Mansour, F.A., Egypt. J. Bot. Streptomyces coeruleovinaceus, Anew Species of Streptomycetes, Produces Anew Antifungal Antibiotic “Vinaceomycin22(1): 1-12,1979.

[22] Mansour, F.A. and Shady, M.A. Factors Influencing the Activity and Production of Nitrate Reductase Enzyme by Local Isolates of Streptomyces. Egypt. J. Bot. 27(1-3): 105- 117, 1984.

[23] MANSOUR,F.A. STUDIES ON THE PROTEOLYTIC ENZYMES OF SOMEACTINOMYCETES.PROC. 2ND CONF.AGRIC.BOT. SCI. MANSOURA PP. 163-193, 1985.

[24] Mansour, F.A. and Mashaly, I.A.. Studies on the Relationship Between the Biological Activities of Streptomycetes and Their Occurrence and Distribution in Egyptian Soil Beitrage Biol. Pflanzen. 66: 353- 365,1985.

[25] Tresner, H.D.; Hayes, J.A. and Backus,E.J. Differential Tolerance of Streptomycetes to Sodium Chloride as Ataxonomic Aid Appl. Microbiol. 16: 1134, 1968.

[26] Lechevalier, H.A. and Lechevalier, M.P. , Chemical Composition as Acriterion in the Classification of Aerobic Actinomycetes Int. J. Syst. Bacteriol. 20: 435- 443, 1970.

[27] Nonomura, H. Key for the Classification and Identification of 458 Species of Streptomycetes Included in the ISP J. Fermentation and Technology.52: 78-92, 1974.

[28] Shirling, E.B. and Gottlieb, D. Cooperative Description of Type Cultures of Streptomyces. II. Species Description from the First Study Int. Syst. Bacteriol. 18: 69-189, 1968a.

[29] Shirling, E.B. and Gottlieb, D. Cooperative Description of Type Cultures of Streptomyces. III. Additional Species Description from First and Second Studies. Int. J. Syst. Bacteriol. 18: 279-392, 1968b.

[30] Shirling, E.B. and Gottlieb, D. Cooperative Description of Type Cultures of Streptomyces. IV. Species Description from Second, Third and Fourth Studies Int. J. Syst. Bacteriol. 19: 391-512, 1969.

[31] Shirling, E.B.a nd Gottlieb, D. Type Cultures of Streptomyces. V. Additional Descriptions Int. J. Syst. Bacteriol. 22: 265-394, 1972.

[32] Bergey’s Manual of Systematic Bacteriology The Actinomycetes. eds. Williams, S.T.,Sharp, M.E. and Holt, J.G. The Williams and Wilkins Company, U.S.A. pp. 2344-3330, 1989.

[33] Siddiq, A. A. N. Studies on the Effeiciency of Antibiotic and Enzymes Produced by Locally Isolated Streptomyces M. Sc. Thesis,Biology Dept., King Abdulaziz Univ. Faculty of Sci, Saudi Arabia,1995.

[34] Alkahtani,M.T. Effects of Antibiotics Produced by Actinomycetes Isolated from Riyadh Region on Some Fungi Ph. D. Thesis, Botany Dept. Girls College of Education, Riyadh, Saudi Arabia, 2005.

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