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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 7    
Website: http://www.ijser.org
scirp IJSER >> Volume 3,Issue 7,July 2012
Bacterial Surface Display using Outer Membrane Proteins as Anchoring Sites
Full Text(PDF, )  PP.258-261  
Sharadwata Pan and Michael K. Danquah
—Anchoring site, bacteria, metalloprotein, outer membrane protein, surface display
Surface display of heterologous proteins or polypeptides on the surface of bacteria has gained momentum in recent years. Until recently, arrays of anchors or carriers have been identified for displaying diverse passenger proteins on the surface of Escherichia coli, majority of these involving the outer membrane proteins (OMPs). The reason for opting outer membrane proteins lies mainly in its ability to withstand the incorporation of large libraries of novel polypeptides, without a significant loss in steadiness. In this context, a thorough understanding of the underlying genetic mechanism of the OMP-mediated surface display is necessary. In this mini-review, we attempt to do the same and also compare the OMPs from two commonly used and available bacteria. The far reaching consequence of this lies in efficient surface display of imaginative and innovative polypeptides with applications ranging from bioremediation, immunology to vaccine development.
[1] S. Pan and M.K. Danquah, ―Cell Surface Display,‖ Biodiversity, A. Sofo, ed., Croatia: InTech, pp. 63-74, 2011.

[2] S.Y. Lee, J.H. Choi and Z. Xu, ―Microbial Cell-Surface Display,‖ Trends Biotechnol, vol. 21, no. 1, pp. 45–52, 2003.

[3] E. van Bloois, R.T. Winter, H. Kolmar and M.W. Fraaije, ―Decorating Microbes: Surface Display of Proteins on Escherichia coli,‖ Trends Biotechnol, vol. 29, no. 2, pp. 79-86, 2011.

[4] C.H. Wu, A. Mulchandani and W. Chen, ―Versatile Microbial Surface Display for Environtal Remediation and Biofuel Production,‖ Trends Microbiol, vol. 16, no. 4, pp. 181-188, 2008.

[5] G.S. Verhoeven, S. Alexeeva, M. Dogterom and T. den Blaauwen, ―Differential Bacterial Surface Display of Peptides by The Transmembrane Domain of OmpA.,‖ PLoS One, vol. 4, no. 8, e6739, 2009.

[6] J.H. Baek, M.-J. Han, S.H. Lee and S.Y. Lee, ―Enhanced Display of Lipase on The Escherichia coli Cell Surface, Based On Transcriptome Analysis,‖ Appl. Environ. Microbiol, vol. 76, no. 3, pp. 971–973, 2009.

[7] J.J. Rice and P.S. Daugherty, ―Directed Evolution of a Biterminal Bacterial Display Scaffold Enhances The Display of Diverse Peptides,‖ Protein Eng. Des. Sel, vol. 21, no. 7, pp. 435–442, 2008.

[8] P. Samuelson, E. Gunneriusson, P.-Å. Nygren and S. Ståhl, ―Display of proteins on bacteria,‖ J. Biotechnol, vol. 96, no. 2, pp. 129–154, 2002.

[9] G.E. Schulz, ―The Structure of Bacterial Outer Membrane Proteins,‖ Biochim. Biophys. Acta, vol. 1565, no. 2, pp. 308–317, 2002.

[10] M.-X. He, H. Feng and Y.-Z. Zhang, ―Construction of a Novel CellSurface Display System for Heterologous Gene Expression in Escherichia coli by Using an Outer Membrane Protein of Zymomonas mobilis as Anchor Motif,‖ Biotechnol. Lett, vol. 30, no. 12, pp. 2111–2117, 2008.

[11] D.F. Shriver and P.W. Atkins, Inorganic Chemistry (Chapter 19, Bioinorganic chemistry), Third edition, UK: Oxford University Press, 1999.

[12] J.D. Kopple and M.E. Swendseid, ―Evidence That Histididne is an Essential Amino Acid in Normal and Chronically Uremic Man,‖ J. Clin. Invest, vol. 55, no. 5, pp. 881-891, 1975.

[13] D.H. Baker and G.L. Czarnecki-Maulden, ―Pharmacologic Role of Cysteine in Amelietoring or Exacerbating Mineral Toxicities,‖ J. Nutr, vol. 117, no. 6, pp. 1003-1010, 1987.

[14] W.S. Willett, S.A. Gillmor, J.J. Perona, R.J. Fletterick and C.S. Craik, ―Engineered Metal Regulation of Trypsin Specificity,‖ Biochemistry, vol. 34, no. 7, pp. 2172–2180, 1995.

[15] Z. Xu, and S.Y. Lee, ―Display Of Polyhistidine Peptides On The Escherichia coli Cell Surface By Using Outer Membrane Protein C as an Anchoring Motif,‖ Appl. Environ. Microb, vol. 65, no. 11, pp. 5142– 5147, 1999.

[16] N. Cruz, S.L. Borgne, G.H. Chavez, G. Gosset, F. Valle and F. Bolivar, ―Engineering The Escherichia coli Outer Membrane Protein OmpC for Metal Bioadsorption,‖ Biotechnol. Lett, vol. 22, no. 7, pp. 623-629, 2000.

[17] T. Mizunol, M.Y. Chou and M. Inouye, ―A Comparative Study on the Genes for Three Porins of the Escherichia coli Outer Membrane,‖ J. Biol. Chem, vol. 258, no. 11, pp. 6932-6940, 1983.

[18] D.R. Lee and C.A. Schnaitman, ―Comparison of Outer Membrane Porin Proteins Produced by Escherichia coli and Salmonella typhimurium,‖ J. Bacteriol, vol. 142, no. 3, pp. 1019-1022, 1980.

[19] E. Gasteiger, A. Gattiker, C. Hoogland, I. Ivanyi, R.D. Appel and A. Bairoch, ―ExPASy: The Proteomics Server for In-depth Protein Knowledge and Analysis,‖ Nucleic Acids Res, vol. 31, no. 13, pp. 3784- 3788, 2003.

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