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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 6    
Website: http://www.ijser.org
scirp IJSER >> Volume 3,Issue 6,June 2012
Comparative Studies on the Prevalence of Bacterial Population and Physicochemical Analysis in Raw and Pasteurised Milk with special reference to Honey as a Natural Preservative
Full Text(PDF, )  PP.879-886  
V.Karthikeyan, S.Ramya, Reshma.V.K
Raw milk, Pasteurised milk, Microbiological, Physicochemical analysis, Proteolytic, Lipolytic, Natural Preservative
In this research, fifteen raw milk samples from different areas and ten pasteurized milk samples of various brands were collected and compared based on microbiological tests and physicochemical analysis. The milk samples were plated on nutrient agar medium and the isolates were then cultured on different media (EMB Agar, McConkey Agar, Skim Milk Agar). These cultured bacterias were then subjected to confirmative tests. The bacterias isolated were Escheritia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus. The milk samples were further subjected to physico-chemical analysis in which Fat content, Clearance Lactometric Reading, Solid Non Fat and Acidity were calculated. The quality of the milk samples were then analysed by Methylene Blue Reduction Test and Clot on Boiling Test.The isolated bacterias were also tested for their proteolytic and lipolytic property and were also subjected to catalase tset. Further the effect of honey as a natural preservative of milk was also analysed by studying the dilution of honey at which the maximum zone of inhibition was observed
1. Franciosi E, De Sabbata G, Gardini F, Cavazza A, Poznanaski E (2011) ‘Changes in psychrotrophic microbial population during milk creaming to produce Grana Trentino Cheese’- Food Microbial Feb 28(1).

2. Ammara Hassan, Imran Amjad and Shahid Mahmood (2009) ‘Microbiological and physicochemical analysis of different UHT milks available in market- African Journal of Food Science Vol. 3(4).pp. 100-106.

3. Jeffrey T.LeJeune and Paivi J. Rajala (2009) -Schultz ‘ Unpasteurised Milk: A continued public health threat’- Clinical Infectious Diseases 48:93-100.

4. Laszlo Varga (2007) ‘ Microbiological quality of commercial dairy products’ – A.Mendez-Vilas(Ed).

5. N S A Krushna, A Kowsalya, S Radha and R B Narayanan (2005) ‘ Honey as a Natural Presrvative of Milk’- Indian Journal of Experimental Biology, Vol.45, pp.459-464.

6. Braun, P. and Fehlhaber, K. (2002). Combined effect of temperature, aw and PH on enzymatic activity of spoilage causing bacteria. Milchwissenschaft. 57(3): 134 – 136.

7. Burdova, O., Barahova, M., Laukova, A., Rozanska, H. and Rola, J.G. (2002). Hygiene of pasteurized milk depending on psychrotrophic microorganisms. Bull. Vet. Inst. Pulawy. 46:325 – 329.

8. Jung, W.( 2002). Bacterial Flora in raw milk views of dairy personnel. DMZ. Lebensittel – Industrie – und – Milchwirtschaft. 123(10): 24 – 30.

9. Prabha, R., Vijaya, G.V., Narayana reddy, K.V. and Bhat, G.S. (2002). Enhancement of functionality of milk proteins by extra cellular proteinases isolated from psychrotrophic organisms in milk. Indian J. Dairy Sci., 55(2): 70 – 73.

10. Sundararaj, T. (2002). Microbiology Laboratory Manual. pp # 218.

11. Pandey, R. and prasad, J.(2001). Influence of treatments of washing of hind quarters and udder on bacterial quality of raw milk. Bioved. 12(1-2):91 – 94.

12. Abdou, S.M., Dawood, A.H., Montasser, E.A. and Ismail, E.E.A. (2001). Evaluation of UHT milk during storage. In: 8th Egyptian conference for Dairy science and Technology held at the International Agriculture centre, Cairo, Egypt, Research papers (I): 149 – 162.

13. Canigova, M. and Benczova, E. (2001). The microflora changes of raw milk during its refrigerated storage. ActaFytotechnica-et-zootechnica. 4(4): 104 – 106.

14. Dawood, A.H., Abdou, S.M., Montasser, E.A. and Ismail, E.A. (2001). Microbiological evaluation of Egyptian ulta-high temperature (UHT) milk. In: 8th Egyptian conference for Dairy science and Technology held at the International Agriculture centre, Cairo, Egypt, Research paper (I): 133 – 147.

15. Geetha, R. and Prasad, V. (2001). Studies on the performance of cultures of lactic acid bacteria in lactose hydrolysed buffalow skim milk. Cheiron. 30(3-4): 81 – 84.

16. Kirin, S. (2001). The effect of cooling procedure on the characteristics and quality of raw milk. Mijekarstvo. 51(2): 151 – 159.

17. Mehanna, N.M., Moussa, M.A.M. and Ahwall, R. (2001). Improving quality of milk during its cold storage. Egyptian J. Dairy sci.,. 29(1): 9 – 18.

18. Carvalho, F. and Luchese, R.H. (2000). Profile of raw milk spoilage microbiota important for UHT milk processing. Anais do XVII congresso Nacional de Laticinios. Perspectivas Advances em Laticinios. 54(318): 188 – 195.

19. Kolakowski, P., Reps. A. and Fetlinski. A. (2000). Microbial quality and some physicochemical properties of high pressure – processed cow milk. Polish journal of food and Nutrition Sciences. 9(4): 19 – 26.

20. Lira, F.C., Oria, M., Hayes, K.D. and Nielsen, S.S. (2000). Effect of Psychrotrophic bacteria and of an isolated protease from Pseudomonas fluorescens M315 on the plasmin system of fresh milk. J. Dairy Sci., 83: 2190 – 2199.

21. Mohran, M.A., Fahmy, M.A., Elhoda Hanary, N. and Nanis, H.G. (2000). Influence of lactic acid bacteria starters on the proteolytic activity of thermoduric bacteria isolated from milk. Assiut Journal of Agricultural sciences. 31(1): 261 – 269.

22. Vyletelova, M., Urbanova, E. and Benda, P. (2000). The incidence of psychrotrophic microorganisms in raw cow milk and the effect of proteolytic and lipolytic enzymes on technological traits of milk. Vyzkum-V-Chovu – Skotu. 42(3): 1 – 10.

23. Vyletelova, M. Hanus, O. Urbanova, E. and Kopunecz, P. (2000). The occurrence and identification of psychrotrophic bacteria with proteolytic and lipolytic activity in bulk milk samples on storage in primary production conditions. czech Journal of Animal Science. 45(8): 373 – 383.

24. Wiedmann, M., Weilmeier, D., Dineen, S., Ralyea, R. and Boor, K.J. (2000). Molecular and phenotypic characterization of Pseudomonas Isolated from milk. Applied and Environmental Microbiology. pp # 2085 – 2095.

25. Cappuccino, J.G. and sherman, N. (1999). Microbiology A Laboratory manual. Benjamin / Cummings publishing company, New York. pp # 477.

26. Jayarao, B.M. and Wang, L. (1999). A study on the prevalence of Gram negative bacteria in Bulk tank milk. J. Dairy Sci., 82:2620 – 2624.

27. Meisel, H. and Backelmann, W. (1999). Bioactive peptides encrypted in milk proteins, proteolytic activation and thropho-functional properties. AntonievanLeeuwenhoek. 76(1- 4): 207 – 15.

28. Santos, E.de.S., Carvalho, Ep.de. and Abrev, L.R. de. (1999). Psychrotrophic bacteria consequences of their presence in milk and cheeses. Boletim – da – Sociedade – Braisileria – de – ciencia – e – Techno – logia – de – Alimentos. 33(2): 129 – 138.

29. Srilakshmi, B. (1999). Food science. New age International private limited publishers, Delhi. pp # 521.

30. Patke, D. and Dey, S. (1998). Proteolytic activity from a thermophilic Streptomyces megasporus strain SDP4. Letters in applied microbiology. 26: 171 – 174.

31. Ibrahim, S.A. (1998). Agglutination Behavior of Mesophilic starter cultures as a function of proteolysis. J. Food prot., 61(7): 855 – 858.

32. Griswold, K.E. and Mackie, R.I. (1997). Degradation of protein utilization of the Hydrolytic products by a predominant Ruminol bacterium, Prevotella ruminicola B14. J. Dairy Sci., 80:167 – 175.

33. Huang – chienJung. and Huang, C.J. (1996). Effect of proteolytic products produced by other lactic acid bacteria in milk on the growth of Bifidobacterium longum. Journal of the chinese society of Animal science. 25(2): 221 – 230.

34. Aslam, M. and Hurley, W.L. (1996). Proteases in Milk. Illinois Dairy Report Home Page. 1 – 3

35. Celestino, E.L., Iyer, M. and Roginski, H. (1996). The effects of refrigerated storage on the quality of raw milk. Australian J. Dairy Tech., 51(2): 59 – 63.

36. Stancheva, N. and Petrova, N. (1995). Technological conditions for cleaning milking machines for ewes using a combined alkaline detergent, and their influence on the microbiological quality and biochemical properties of milk. Zhivotnovdhi – Nauki. 32(5 – 8): 325 – 328.

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