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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 7    
Website: http://www.ijser.org
scirp IJSER >> Volume 3,Issue 7,July 2012
Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Casia occidentalis (L) Plant Extract
Full Text(PDF, )  PP.490-493  
Venkanna Lunavath and Estari
: HIV, Casia occidentalis, PBMCs, HIV-1 RT, Cytotoxicity.
Human immunodeficiency virus type-1(HIV-1) is the cause of acquired immune deficiency syndrome (AIDS), a major human viral disease with about 33.2 million people infected worldwide. The current treatment for HIV/AIDS is called Highly Active Anti-Retroviral Therapy (HAART) and is a combination of anti-HIV reverse transcriptase inhibitors and protease inhibitors. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. In the current study, we characterized a panel of extracts of traditional medicinal plants for their activities against HIV-1 replication. The aim of the present study was to evaluate the invitro anti- HIV activity of Casia occidentalis plant extracts. Extracts were prepared from dried leaves in n-hexane, ethyl acetate and n butanol. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts by MTT assay using PBMCs isolated from whole blood. HIV-1 RT inhibition activity of the all solvent extracts of Casia occidentalis was determined by a RetrsoSys HIV-1 RT activity kit (Innovagen, Sweden). The leaves of Casia occidentalis extracts are shows anti-HIV-1 activity and this plant has great potential for developing useful drugs.
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