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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 7    
Website: http://www.ijser.org
scirp IJSER >> Volume 3,Issue 7,July 2012
Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Casia occidentalis (L) Plant Extract
Full Text(PDF, )  PP.490-493  
Author(s)
Venkanna Lunavath and Estari
KEYWORDS
: HIV, Casia occidentalis, PBMCs, HIV-1 RT, Cytotoxicity.
ABSTRACT
Human immunodeficiency virus type-1(HIV-1) is the cause of acquired immune deficiency syndrome (AIDS), a major human viral disease with about 33.2 million people infected worldwide. The current treatment for HIV/AIDS is called Highly Active Anti-Retroviral Therapy (HAART) and is a combination of anti-HIV reverse transcriptase inhibitors and protease inhibitors. The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population in the world. This reality has urgently called for the need to develop inexpensive alternative anti-HIV/AIDS therapy. In the current study, we characterized a panel of extracts of traditional medicinal plants for their activities against HIV-1 replication. The aim of the present study was to evaluate the invitro anti- HIV activity of Casia occidentalis plant extracts. Extracts were prepared from dried leaves in n-hexane, ethyl acetate and n butanol. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts by MTT assay using PBMCs isolated from whole blood. HIV-1 RT inhibition activity of the all solvent extracts of Casia occidentalis was determined by a RetrsoSys HIV-1 RT activity kit (Innovagen, Sweden). The leaves of Casia occidentalis extracts are shows anti-HIV-1 activity and this plant has great potential for developing useful drugs.
References
1) Husain A, Virmani OP, Popli SP, Misra LN, Gupta MM, Srivastava GN, Abraham Z and Singh AK, Dictionary of Indian Medicinal Plants, Central Institute of Medicinal and Aromatic Plants, Lucknow, 1992, pp. 61, 389.

2) Chinsembu KC (2009). Model and experiences of initiating collaborationwith traditional healers in validation of ethnomedicines for HIV/AIDSin Namibia. J. Ethnobio. Ethnomed. 5:30 doi:10.1186/1746-4269-5-30.

3) Wang, J.H., Tam, S.C., Huang, H., Ouyang, D.Y., Wang, Y.Y., Zheng, Y.T.,2004a. Site-directed PEGylation of trichosanthin retained its antiHIVactivity with reduces potency in vitro. Biochemical and BiophysicalResearch Communications 317, 965–971.

4) Elaya RA, Vijayalakshmi M, Devalarao G (2009). Acorus calamus Linn.:chemistry and biology research. Res. J. Pharm. Technol., 2: 256–261.

5) Akanitapichat P, Kurokawa M, Tewtrakul S, Pramyothin P,Sripanidkulchai B, Shiraki K, Hattori M (2002). Inhibitory activities ofThai medicinal plants against herpes simplex type 1, poliovirus type1, and measles virus. J. Tradit. Med., 19: 174– 180.

6) Harris JC, Cottrell SL, Plummer S, Lloyd D (2001). Antimicrobialproperties of Allium sativum (garlic). Appl. Microbiol. Biol., 57: 282–286.

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