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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 2    
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scirp IJSER >> Volume 3,Issue 2,February 2012
Study of Inoculum size, Incubation temperature and Nucleic Acid Concentration on the Single Cell Protein produced by using Soymilk residue (Okara)
Full Text(PDF, )  PP.351-354  
Author(s)
Mahmood Khan Yousufi
KEYWORDS
: Inoculum size, Incubation temperature, Nucleic acid, SCP, Okara.Rhizopus oligosporus and Aspergillus oryzae.
ABSTRACT
Okara, a soymilk residue is produced in huge quantities in soymilk industries. In the present investigation three combinations of Okara and wheat grits where prepared in the ratio of 3:1 (60% moisture content), 1:1 (40% moisture content), 1:3 ( 25 % moisture content) respectively. The combinations were inoculated with two fungal species viz. Rhizopus oligosporus and Aspergillus oryzae, with an inoculum size of 1.0 x I0 3 , 1.0 x I0 4 and 1.0 x I0 5 cfu per g substrate. The combinations were incubated at different temperatures i.e. 200 c, 250 c and 300 c. The combinations were finally analyzed for the effective inoculum size, incubation temperature and nucleic acid concentration. The results obtained depict that the best inoculum size for maximum SCP yield was 1.0 x I0 3 cfu per g substrate. The best incubation temperature was found to be 250 c. The maximum nucleic acid concentration was found to be 249 mg for R. oligosporus and 260 mg for A. oryzae.
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