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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 9    
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scirp IJSER >> Volume 2, Issue 9, September 2011
Antagonistic activity evaluation of Eucalyptus camaldulensis essential oil in control of algae and associated microorganisms
Full Text(PDF, 3000)  PP.  
Author(s)
Ali M. Najem, Abdul Latif M. Jawad and Ayyad W. Raof
KEYWORDS
control algae,antagonistic activity ,eucalyptus camaldulensis
ABSTRACT
These instructions give you guidelines for preparing papers for IJSER JOURNALS. Use this document as a template if you are This study includes isolation, purification, and identification of algae from different environmental places (Tigris River and canal around Al- Jadriah) during February 2008. Nine unialgal cultures were obtainedwhich included 4 species of blue-green algae, 4 species of green algae, and 1 species of diatoms. However, contaminant bacteria and fungi were isolated from 4 of these algal cultures which were Anabaena circinalis, Microcystis aeruginosa, Scenedesmus quardicauda and Chlorella vulgaris. The contaminant bacteria included nine different species which were Stenotrophomonas maltophilia and Bacillus polymyxa which isolated from all the selected algal cultures, while the rest Kocuria varians/rosa, Aerococcus viridians, Micrococcus sp., Staphylococcus auricularis, Staphylococcus capitis, Kocuria kristanae and Staphylococcus cohnii varied in their presence and absence in these algal cultures. The contaminant fungi of the four algal cultures were Aspergillus flavus which was isolated from M. aeruginosa culture, Asp.niger isolated from C. vulgaris culture , Cryptococcus albidus that isolated from S. quardicauda culture, whilst Penicillium sp. isolated from A. circinalis culture. However, Eucalyptus camaldulensis essential oil was extracted and subsequently showed highly activity when tested against six species of the isolated algae and against the isolated contaminant bacteria and fungi species. Chemical composition of the extracted oil was analyzed by HPLC (High Performance Liquid Chromatography).
References
1. Abedin, R.M.A. and H.M.T. Taha. 2008. Antibacterial and antifungal activity of Cyanobacteria and green microalgae. Evaluation of medium components by Plackett. Burman design for antimicrobial activity of Spirulina platensis. Gobal J. Biotech. and Biochem. 3(1): 22. 31.

2. Adeniyi, B.A. and O.O. Ayepola. 2008. The Phytochemical Screening and Antimicrobial Activity of Leaf Extracts of Eucalyptus camaldulensis and Eucalyptus torelliana (Myrtaceae). Research Journal of Medicinal Plant. 2, pp: 34.38.

3. Akin, M.; A. Aktumsek and A. Nostro. 2009. Antibacterial activity and composition of the essential oils of Eucalyptus camaldulensis Dehn. and Myrtus communis L. growing in Northern Cyprus. African Journal of Biotechnology Vol. 9 (4), Pp. 531.535.

4. Al.Chaesi, S.A.S. 2008. Effect of Myrtus communis essential oil on growth and activity of several pathogenic bacterial types and Candida albicans yeast. Om Salma Journal for Siences, 5: 8. 12. (In Arabic)

5. Allen, M. 1968. Simple conditions for the growth of unicellular blue.green algae on plates. Journal of Phycology, 4:1.4.

6. Alma, M.H.; S. Nitz; H. Kollmannsberger ; M. Digrak ; F.T. Efe and N. Yilmaz . 2004. Chemical composition and antimicrobial activity of the essential oils from the gum of Turkish pistachio (Pistacia vera L.). J .Agric. Food Chem. 52(12):3911–4.

7. Begum, S.H.; S.M.M.H. Majumder; M.A. Majid and M. M. Hossain. 2005. Isolation, Characterization and Study of the Physical, Chemical and Anti Microbial Properties of Fat Obtained from Eucalyptus (Eucalyptus camaldulensis Dehnh) Leaf. European Journal of Scientific Research, Vol 10, 3.

8. Benson, H.J. 2002. Microbiological Applications Laboratory Manual in General Microbiology. New York. McGraw.Hill companies.

9. British Pharmacopoeia, 1963. Determination of Volatile Oil in Drugs. The Pharmaceutical Press, London.

10. Cooradi, M.G.; G. Orbi; H.M. Abdel Moneam; A. Torelli and M. Bassi. 1999. Exudates from the wild type and a CR.tolerant strain of Scenedesmus acutus influence differently CR (VI) toxicity to algae. Science Direct, vol 37, Pp: 3019.3025.

11. Cowan, M.M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12:564–582.

12. Desikachary, T.V. 1959. Cyanophyta. Academic press. New York.

13. Ghalem, B.R and B. Mohamed. 2008. Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. African Journal of Pharmacy and Pharmacology Vol. 2(10). Pp. 211.215.

14. Greca, M. D.; M. Ferrara; A. Fiorentino; P. Monaca, and L. Previtera.1998. Antialgal compounds from Zantedeschia aethiopica. Phytochemistry, 49: 1299.1304.

15. Hallegraeff, G. M.; D. M. Andersen and A.D. Cembella. 2003. Manual on Harmful Marine Microalgae. UNESCO Publishing, Paris.

16. Jawad, A.L.M.1982. Interaction between cyanobacteria and other micro.organisms. Ph.D.Thesis.Liverpool University. England.

17. Kassim, T.I.; H. AL.Saadi and N.A. Salman. 1999. Production of some phytoplankton and zooplankton and their use as live food for fish larva. Iraqi J. Agric. Proc. of 2nd Sci. Confer., 4(5): 188.201.

18. Kobaisy, M.; R. Mario; R. Tellez; C. L. Webber; F. E. Dayan; K. K. Schrader and D. E. Wedge. Phytotoxic and Fungitoxic Activities of the Essen.tial Oil of Kenaf (Hibiscus cannabinus L.) Leaves and Its Composition. Journal of Agiculture and Food Chemistry, 49(8): 3768.3771.

19. Miline, L.J.R. 1996. Fungi In: Practical Medical Microbiology edited by Collee, J.F. Churchill, Livingstone.

20. Nakai, S.; Y. Inoue and M. Hosomi. 2001. Algal growth inhibition effects and inducement modes by plant.producing phenols. Water Res., 35 (7) 1855.1859.

21. Nakai, S.; Y. Shingo and H. Massaki. 2004. Anti.cyanobacterial fatty acids released from Myriophyllum spicatum.Hydrbiologia, 543:71.78.

22. Nicholas, S.A. 1973. The effects of harvesting aquatic macrophytes on algae. Transactions of the Wisconsin Academy of Sciences, Arts and Letters, 61: 165.172.

23. Perez, L.; M. Pauli and P.Bazequre.1990.Antibiotic assay by the agar well diffusion method. Journal of Actabiology, 15: 113. 115.

24. Prescott, G.W. 1973. Algae of the Western Great Lakes Area. OHO Koeltz. Science publishers. Germany.

25. Reichling, J. 1999. Plant.microbe interaction and secondary metabolites with antiviral, antibacterial and antifungal properties; in Wink M (ed): Functions of Plant Secondary Metabolites and Their Exploitation in Biotechnology. Sheffield, Sheffield Academic Press, 1999, pp 187–273.

26. Reichling, J.; U. Suschke ; J. Schneele and H.K. Geiss. 2006. Antibacterial activity and irritation potential of selected essential oil components – structure.activity relationship. Nat.Prod. Commun., 1:1003–1012.

27. Rice, E. L. 1984. Allelopathy, 2nd ed. Academic Press, Ltd., London, United Kingdom.

28. SAS. 2004. SAS / STAT Users Guide for Personal Computers. Release 6.12. SAS Institute Inc., Cary, NC., USA. (SAS = Statistical Analysis System).

29. Shapiro, J.; V. Lamarra and M. Lynch. 1975. Biomanipulation: An ecosystem approach to lake restoration. In: Brezonick, P.I. and J.L. Fox (eds.), Water quality management through biological control. Department of Environmental Engineering Scinces. University of Florida, Gaenisville, Pp: 85.96.

30. Sivonen, K. and G. Jones. 1999. Cyanobacterial toxins, pp: 41–111. In : Chorus and J. Bartram (ed.), Toxic cyanobacteria in water. E&FN Spon,London, Great Britain.

31. Stein, J.R.1973. Handbook of phycological methods: Culture methods and growth measurements. Cambridge University Press. Cambridge.

32. Wang, S.Y.; C. Wu; F. Chu; S. Chien ; Y. Kuo ; L. Shyur; and S. Chang. 2005. Chemical composition and antifungal activity of essential oil isolated from Chamaecyparis formosensis Matsum. Wood. Holzforschung, 59, Pp. 295–299.

33. Yang, W.; J.S. Lu; H.Y. Li; X.L. Zang and Y.Z. Oi. 2009. Inhibition of the Growth Alexadrium tamarense by Algicidal Substances in Chinese Fir (Cunninghamia lanceolata). Bull. Environ. Contam. Toxicol. 83: 537.541

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