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International Journal of Scientific and Engineering Research
ISSN Online 2229-5518
ISSN Print: 2229-5518 10    
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scirp IJSER >> Volume 2, Issue 10, October 2011 Edition
Alkaline Phosphatase Activity during Homogenisation of Hepatopancreatic Tissues of Shrimps using Sodium acetate, KCl solution, Tris-HCl and Glycine-NaOH buffer.
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Kathyayani Puttige, Krishna Prasad Nooralabettu
Alkaline phosphatase, Shrimps, Black tiger shrimp, Indian shrimp, White shrimp, Brown shrimp, tiny shrimp, Buffer,Homogenisation
Hepatopancreatic tissues of shrimps, Penaeus monodon, Penaeus indicus, Metapenaeus monocerus, Solenocera Choprai and Parapenaeopsis stylifera was homogenized at 3000 rpm/10 min using various buffers such as 0.5M Sodium acetate buffer, 2M KCl solution, Deionised water, 0.1M Tris-HCl buffer and 0.1M Glycine-NaOH buffer of pH 5.5, 7.0, 7.4, 8.4 and 9.5, respectively. Alkaline phosphatase activities in each of these tissue homogenates were assayed using p-nitrophenylphosphate either as a substrate in respective homogenisation buffer or 2-amino 2-methyl 1-propanol buffer, as the liberation of p-nitrophenol/min at 37 oC/L of homogenate. Enzyme activity increased by more than 1.5 folds as the assay buffer changed from respective homogenisation buffer to AMP-buffer. Lowest activity was observed in 0.5M Sodium acetate and highest activity was observed in 0.1M Tris-HCl buffer. Increase in pH of the buffer increased the activity of alkaline phosphatase, but Glycine-NaOH buffer even at pH 9.5 did not favour the activity compared to Tris-HCl buffer
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