Toxicity Evaluation of Cnidoscolus Aconitifolius on Female Albino Wistar Rats [ READ ]
Chukwuemeka, Obinna Godfrey, Nwankpa P.; Uloneme, G. C.; Etteh, C. C.; Ben-Udechukwu, Chioma; Okafor, P. N.
Toxicity evaluation of Cnidoscolus aconitifolius on female albino Wistar rats was investigated by monitoring toxicity indices such as serum glutathione, antioxidant capacity and elevated cyanide in serum and urine and elevated serum thiocyanate and nitrite. Feeding of female albino Wistar rats with Cnidoscolus aconitifolius containing 12.7 mg CN-1kg-1wt/wt gave the following results; group D (animals fed with 50% Cnidoscolus leaves) had the highest mean concentration value (538.19 ± 39.28) for total cyanide (TCN)(µg/ml) in urine while the control group (animals fed with only commercial feed) had the least concentration value(29.65 ± 4.42) in urine and for free cyanide (FCN)(µg/ml) in urine, group D had 53.39 ± 1.66 while group A had 0.7 ± 0.68. But for serum total cyanide (µg/ml), the control group had the highest mean concentration value of 3.079 ± 1.52 and 1.266 ± 0.1 for serum free cyanide (FCN)(µg/ml) whereas group D had 0.358 ± 0.102 for serum total cyanide and 0.106 ± 0.08 for serum free cyanide. Total antioxidant capacity of the analysis was also observed using Sorbo method (1953) with respect to thiocyanate ion concentration in serum (µm/ml). Serum thiocyanate concentration was found to occur highest in group D with 3.50 ± 0.27 and the control group had the least with 0.15 ± 0.03. Decrease in whole blood glutathione (an important biological antioxidant) was also observed and group D contained the least mean value with -0.018 ± 0.008 (µg/ml) while the control group had the highest with 0.260 ± 0.003 (µg/ml). Increase in nitrite (N-nitrosamine precursor) was also observed. Group D had 19.94 ± 2.00 mean concentration value (µm/ml) whereas the control group had the least with 9.62 ± 1.17 (µm/ml). The toxicological implications of these findings are discussed.
Chukwuemeka, Obinna Godfrey, Nwankpa P.; Uloneme, G. C.; Etteh, C. C.; Ben-Udechukwu, Chioma; Okafor, P. N.
Toxicity evaluation of Cnidoscolus aconitifolius on female albino Wistar rats was investigated by monitoring toxicity indices such as serum glutathione, antioxidant capacity and elevated cyanide in serum and urine and elevated serum thiocyanate and nitrite. Feeding of female albino Wistar rats with Cnidoscolus aconitifolius containing 12.7 mg CN-1kg-1wt/wt gave the following results; group D (animals fed with 50% Cnidoscolus leaves) had the highest mean concentration value (538.19 ± 39.28) for total cyanide (TCN)(µg/ml) in urine while the control group (animals fed with only commercial feed) had the least concentration value(29.65 ± 4.42) in urine and for free cyanide (FCN)(µg/ml) in urine, group D had 53.39 ± 1.66 while group A had 0.7 ± 0.68. But for serum total cyanide (µg/ml), the control group had the highest mean concentration value of 3.079 ± 1.52 and 1.266 ± 0.1 for serum free cyanide (FCN)(µg/ml) whereas group D had 0.358 ± 0.102 for serum total cyanide and 0.106 ± 0.08 for serum free cyanide. Total antioxidant capacity of the analysis was also observed using Sorbo method (1953) with respect to thiocyanate ion concentration in serum (µm/ml). Serum thiocyanate concentration was found to occur highest in group D with 3.50 ± 0.27 and the control group had the least with 0.15 ± 0.03. Decrease in whole blood glutathione (an important biological antioxidant) was also observed and group D contained the least mean value with -0.018 ± 0.008 (µg/ml) while the control group had the highest with 0.260 ± 0.003 (µg/ml). Increase in nitrite (N-nitrosamine precursor) was also observed. Group D had 19.94 ± 2.00 mean concentration value (µm/ml) whereas the control group had the least with 9.62 ± 1.17 (µm/ml). The toxicological implications of these findings are discussed.
