Collagen is the main connective protein in the body of an animal predominantly found in the bones and skin of mammals. It is the primary source for gelatin recovery when subjected to a denaturing process at a control temperature. The aim of this study is to extract gelatin by hydrolysis, modified and determining by gravimetrical and scan electron micrograph characteristics of both the unmodified and fatty acid ester modified gelatin; the gelatin extraction percentage yield on a dry basis from collagenous cow bone after 20 hrs hydrolysis is 23.2%. The modified gelatine film subjected to hydrophocity test shows that water vapour transmission capacity of 3.9 x 10-8 gmm-1pa-1hr-1 was experimentally obtained by gravimetry desiccant method which implied that the hydrophobicity of the modified gelatine film is high to a stable accuracy. Comparison of microstructural and morphological properties of unmodified and hydrophobically modified gelatine films shows that a face cubic center (FCC) plane grain boundaries with a fibre internal structure of 1.29µm, 2.4 µm and 7.88 µm and pore average height, thickness area of 0.41µm2, 1.44 µm2 and 103.10 µm2 respectively at a stable frequency were noticed in the unmodified gelatine and this explaine it brittle and surface wettability behavior. The hydrophobically modified gelatine has a rough surface region of a micro-structural morphology of a body cubic center (BCC) micrograph with a semi-crystalline lattice structure due to the present of a rod-like curve amorphous solid and a tetrahedron ice crystal solid on a rough surface region with fibre internal structure of 757.07 nm, 4.18µm, and 14.01µm and pores of average dept, thickness and area of 0.41 µm2, 2.32 µm2 and 22.98 µm2. Roughness of the surface region confirmed it hydrophobicity ability. The modified gelatin obtained from collagenous cow bones formulated an environmentally friendly bioplastizer or binder for explosive synthesis.