Incidence and Detection of Citrus Tristeza Virus in Egypt by ELISA, RT-PCR and real-time PCR (TaqMan®) [ READ ]
Sahar A.Youssef; Elharony, S.B ; Abo El-Abbas, F. M, and A.A.Shalaby
Citrus tristeza virus (CTV) has been previously reported in citrus growing regions of Egypt. Incidence, distribution of CTV and extensive surveys were conducted in citrus growing areas of Qalyubia, Ismailia and Sharkya Governorates during 2012-13 to determine the occurrence of the virus, to evaluate its incidence, to identify and characterize the CTV isolate. A total of 3630 citrus seedlings and trees from nurseries and orchards were sampled from more than 10300 Feddans of citrus in three governments. Leaf samples were collected and symptom intensity of the trees was recorded. Field observations including stem pitting, stunting, vein clearing and leaf curling symptoms revealed the existence of CTV. Double Antibody Sandwich - Enzyme Linked Immunuo-Sorbent Assays (DAS-ELISA), one step RT-PCR and TaqMan real time- RT-PCR were used to index and confirm the existence of CTV in different locations in Egypt. Isolate genotyping was conducted using reverse-transcription polymerase chain reaction (RT-PCR). RT-PCR and the real-time RT-PCR assays developed were more sensitive and less time consuming than DAS-ELISA.
Sahar A.Youssef; Elharony, S.B ; Abo El-Abbas, F. M, and A.A.Shalaby
Citrus tristeza virus (CTV) has been previously reported in citrus growing regions of Egypt. Incidence, distribution of CTV and extensive surveys were conducted in citrus growing areas of Qalyubia, Ismailia and Sharkya Governorates during 2012-13 to determine the occurrence of the virus, to evaluate its incidence, to identify and characterize the CTV isolate. A total of 3630 citrus seedlings and trees from nurseries and orchards were sampled from more than 10300 Feddans of citrus in three governments. Leaf samples were collected and symptom intensity of the trees was recorded. Field observations including stem pitting, stunting, vein clearing and leaf curling symptoms revealed the existence of CTV. Double Antibody Sandwich - Enzyme Linked Immunuo-Sorbent Assays (DAS-ELISA), one step RT-PCR and TaqMan real time- RT-PCR were used to index and confirm the existence of CTV in different locations in Egypt. Isolate genotyping was conducted using reverse-transcription polymerase chain reaction (RT-PCR). RT-PCR and the real-time RT-PCR assays developed were more sensitive and less time consuming than DAS-ELISA.
