Hyperprolactinemia induced histological and cytoskeletal vimentin alterations in mice thyroid glands [ READ ]
Nabila I. El-Desouki, Mohamed L. Salem, Mohamed Nasef. and Faten M. Abdallah
The present investigation is planned to demonstrate histological and immunohistochemical detection of vimentin of the thyroid gland of hyperprolactinemic adult male mice (Mus musculus) for different durations by using metoclopramide (MCP). Mice were divided into five groups. Group I: control mice group were injected with saline solution i.p. for 10 weeks, groups II, III, IV and V; mice were treated with MCP i.p. in a dose of 2.2 mg/kg/ b.w daily for different durations 2, 4, 7 and 10 weeks, respectively. The results recorded a significant increase in the body weight of mice groups III, IV and V, and a significant increase in the levels of prolactin hormone of groups IV and V. The thyroid gland of the control mice group stained with H &E demonstrated normal appearance of follicles with normal simple cuboidal cells; each cavity is filled with acidophilic colloid. HPRL groups for 2 & 4 weeks (groups II&III) showed histopathological changes include vacuolation of cytoplasm, fusion of some follicles and others are free from colloids.
Nabila I. El-Desouki, Mohamed L. Salem, Mohamed Nasef. and Faten M. Abdallah
The present investigation is planned to demonstrate histological and immunohistochemical detection of vimentin of the thyroid gland of hyperprolactinemic adult male mice (Mus musculus) for different durations by using metoclopramide (MCP). Mice were divided into five groups. Group I: control mice group were injected with saline solution i.p. for 10 weeks, groups II, III, IV and V; mice were treated with MCP i.p. in a dose of 2.2 mg/kg/ b.w daily for different durations 2, 4, 7 and 10 weeks, respectively. The results recorded a significant increase in the body weight of mice groups III, IV and V, and a significant increase in the levels of prolactin hormone of groups IV and V. The thyroid gland of the control mice group stained with H &E demonstrated normal appearance of follicles with normal simple cuboidal cells; each cavity is filled with acidophilic colloid. HPRL groups for 2 & 4 weeks (groups II&III) showed histopathological changes include vacuolation of cytoplasm, fusion of some follicles and others are free from colloids.
