Determination of lipase production and partial purification from local bacterial and micro algal isolates [ READ ]
Dr. Amna M. Ali, Dr. Neihaya H. Zaki and Dr. Geddaa Al-rubaie
Lipase is enzyme capable of catalyzing and synthesis of esters formed from glycerol and long-chain fatty acids. Many types of microorganisms produce lipase, bacterial and algae lipases are important enzymes applications in various industries. Six bacterial and six algae species were collected from soil and research laboratories in Biology department/ Al- Mustansiriyah University, bacterial isolates identified as (Enterobacter aerogenes, Pseudomonas alcaligenes, Aeromonas veronii, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus) and algae species as; (Chroococcus minor, Oscillatoria tunuis, Anabeana sp., Chlorella vulgaris, Nostoc sp.,and Chlorella ellipsodea). All bacterial and algae species were screened the ability for lipase production using plate assay and participation method for partial purification. Results showed a significant production of lipase for most of bacterial species, while very little to no inactivation effect for Aromonas hydrophlia, also there were an obvious production of ps alcaligenes and S. marcescens using plate assay method. A significant production for most of algae speices for lipase enzyme while very little to no inactivation effect for Chroococcus minor. Protein participation by using ammonium sulphate applied to purify the enzyme in 75% saturation. Results demonstrated that S. marcescens bacteria and Chlorella ellipsoidea algae were best isolates for extracellular lipase production, and that crud enzyme has less activity than purified enzyme. Isolation and identify the Lipase enzyme production form different local bacterial and algae isolates species has a great effect on involve this enzyme in the scientific application, because of friendly for environment, non-toxic and no harmful residues.
Dr. Amna M. Ali, Dr. Neihaya H. Zaki and Dr. Geddaa Al-rubaie
Lipase is enzyme capable of catalyzing and synthesis of esters formed from glycerol and long-chain fatty acids. Many types of microorganisms produce lipase, bacterial and algae lipases are important enzymes applications in various industries. Six bacterial and six algae species were collected from soil and research laboratories in Biology department/ Al- Mustansiriyah University, bacterial isolates identified as (Enterobacter aerogenes, Pseudomonas alcaligenes, Aeromonas veronii, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus) and algae species as; (Chroococcus minor, Oscillatoria tunuis, Anabeana sp., Chlorella vulgaris, Nostoc sp.,and Chlorella ellipsodea). All bacterial and algae species were screened the ability for lipase production using plate assay and participation method for partial purification. Results showed a significant production of lipase for most of bacterial species, while very little to no inactivation effect for Aromonas hydrophlia, also there were an obvious production of ps alcaligenes and S. marcescens using plate assay method. A significant production for most of algae speices for lipase enzyme while very little to no inactivation effect for Chroococcus minor. Protein participation by using ammonium sulphate applied to purify the enzyme in 75% saturation. Results demonstrated that S. marcescens bacteria and Chlorella ellipsoidea algae were best isolates for extracellular lipase production, and that crud enzyme has less activity than purified enzyme. Isolation and identify the Lipase enzyme production form different local bacterial and algae isolates species has a great effect on involve this enzyme in the scientific application, because of friendly for environment, non-toxic and no harmful residues.
