There is worldwide interest in the synthesis of nanoparticles. In this study the copper nanoparticle synthesized by biological method, provides a feasible alternative as compared to chemical and physical methods that are expensive and can involve the use of toxic, hazardous chemicals, which may pose biological and environmental risk. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as cost-effective. Aloe vera (Aloe barbadensis) leaf extract is a medicinal agent with multiple properties other than applications exploiting their antibacterial activity. This work reports the biosynthesis of Copper nanoparticle using Aloe barbadensis. The plant extract was prepared with triple distilled water by cold percolation method. The sample was maintained at pH 9 and 1000 C temperature. The preliminary characterization of nanoparticles was done by using Colorimeter at 540nm with the regular 1 hour interval. Confirmatory analyses of synthesized copper nanoparticles were done by using TEM (Transmission electron microscopy), SEM (Scanning electron microscopy) and EDS (Energy Dispersion X-ray Spectrometer). These biosynthesized copper nanoparticles were used in the evaluation of antimicrobial activity that was done by Turbidimetric analysis method against facial skin flora. The detection of the synthesized nanoparticles is observed by Colorimeter at 540 nm. Keywords: Green synthesis, Copper nanoparticles, Cold Percolation, TEM (Transmission Electron microscopy), SEM (Scanning Electron microscopy), EDS (Energy Dispersion X-ray Spectrometer), Antibacterial activity, Skin flora.

TITLE - Plant mediated biological synthesis of copper nanoparticles using Aloe barbadensis peel and its antimicrobial effects on various human skin flora.
AUTHOR - Harsharan kaur
••••••IJSER Edition - July 2021

UNIVERSITY - Guru Nanak Girls College, Model town, Ludhiana (Panjab University, Chandigarh)
GUIDE NAME -
Mrs. Ratika Komal