Isolation and Structural Identification of Compounds with Antioxidant, Nematicidal and fungicidal Activities from Punica granatum L. var. nana [ READ ]
Emam, A.M, Ahmed, M.A.M, Tammam, M.A.A, Hala, A.M. El-Dakar and Zawam,S. Hanaa
Pure compounds have been isolated from methanolic extract of Punica granatum L. var. nana. The crude extract and pure isolated compounds have been tested for their antioxidant, nematicidal and fungicidal activities against three nematode species, i.e. Meloidagyne incognita, Rotylenchulu reniformis and Pratylenchus penetrans as well as three phytopathogenic fungi, i.e Fusarium oxysorum f.sp. lycopersici ,Rhizoctonia solani and Sclerotium rolfsii. The methanolic extract exhibited free radical scavenging activity against DPPH radicals (IC50=25.97µg/ml-1). Activity guided separation resulted in isolation of three active compounds namely; Cyanidin 3,5-diglucoside (IC50=5.2 µg/ml-1), Galloyl glucoside (IC50=4.1 µg/ml-1) and 2-methyl-pyran-4-one-3-O-β-D-glucopyranoside (IC50=5.5 µg/ml-1).
Emam, A.M, Ahmed, M.A.M, Tammam, M.A.A, Hala, A.M. El-Dakar and Zawam,S. Hanaa
Pure compounds have been isolated from methanolic extract of Punica granatum L. var. nana. The crude extract and pure isolated compounds have been tested for their antioxidant, nematicidal and fungicidal activities against three nematode species, i.e. Meloidagyne incognita, Rotylenchulu reniformis and Pratylenchus penetrans as well as three phytopathogenic fungi, i.e Fusarium oxysorum f.sp. lycopersici ,Rhizoctonia solani and Sclerotium rolfsii. The methanolic extract exhibited free radical scavenging activity against DPPH radicals (IC50=25.97µg/ml-1). Activity guided separation resulted in isolation of three active compounds namely; Cyanidin 3,5-diglucoside (IC50=5.2 µg/ml-1), Galloyl glucoside (IC50=4.1 µg/ml-1) and 2-methyl-pyran-4-one-3-O-β-D-glucopyranoside (IC50=5.5 µg/ml-1).
